I have always been used to finding and reading research papers for projects, assignments, or just for knowledge, but, being on the other side of that experience was quite fascinating and honestly, eye-opening. My team and I chose to work with Sangath, a mental health NGO based in Goa. We acted as research consultants, conducted extensive research, and curated a comprehensive research paper on the topic “Impact of the pandemic on social relations between teens”. As this topic was very relatable and subjective, we had to tread the fine line between maintaining objectivity and respecting the personal experiences of all the individual interviews and surveys.
Having to start research from scratch on a topic that does not have many reference materials due to its recency was a challenge, but after scouring the internet for any related literature, conducting several surveys with over 800 responses, interviews with students, professionals, parents, and teachers, we were able to create a research paper that sticks to its objective and effectively incorporates and values the individual experience. Having done all this within a span of a month, all while balancing academics made that research paper even more valuable.
Ms. Bhargavi of Grade 12 Ekya ITPL whose research on infections and infection-causing diseases was published by the American Journal of Applied Biotechnology Research (AJABTR)
This is what Ms. Bhargavi had to say about her experience.
I learned about infections and infection-causing diseases in grade 12 and how and what would get infected when bacteria make it their home. I learned that there are mostly a group of bacteria present on a wound and not just a single one to cause infection. I researched more on this topic to fuel my rising curiosity and found that the group of bacteria or the consortia of bacteria are called biofilms and stay in groups and cover themselves with EPS which results in antimicrobial and antibiotic resistance.
In this project, I learnt that Biofilms are syntrophic consortia of microorganisms that remain adhered on biotic and abiotic surfaces with the help of extracellular polymeric surface (EPS). This does not only provide nourishment to the dwelling cells but also prevents the penetration of any drug molecules resulting in the development of antibiotic/antimicrobial resistance. The emphasis of this project is the usage of alternative therapeutic and natural compounds in the treatment of biofilm-associated infection. This would primarily be on the basis of observing the molecular interaction taking place between the virulent protein and the natural compound.
The phytocompounds in the research were Curcumin( found in Turmeric), Eugenol ( found in clove buds, tulsi leaves, etc), Quercetin( found in onions), Nimbin( found in neem, and Gingerol ( found in ginger). The study concluded that Nimbin showed maximum interaction with the biofilm-forming proteins of S. aureus and P. aeruginosa. This depicts that phytocompounds can be used as alternate therapeutics in the inhibition of the biofilm formed by major organisms.
In this project, I was responsible for carrying out extensive research work in understanding the molecular docking interaction of various phytocompounds with the targeted virulent biofilm-forming proteins of S. aureus and P. aeruginosa. I effectively analyzed the drug-like properties of various phytocompounds and analyzed the antibiotic-resistant genes that are associated with the modeled biofilm organisms as part of the research project.
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